User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/04/28

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April 28th, 2010

I made two restrictions of the plasmid I extracted yesterday with EcoRI and PstI.
I followed the next protocol:
20μL DNA
4μL Buffer 3
1μL each enzyme
1μL BSA
1μL H2O
After incubate 5h30 I made an agarose gel to 8% with the restricted plasmids and the original ones.
Ladder | DNA restricted 4μL | Plasmid 5μL | Ladder

  0     1  2  3  4     5  6  7  8     9      <---Lanes

It seems that this gel it's OK, it showed two bands of 3kb in the lanes from 1 to 4.
(The small piece of 100bp that we expected didn't appear but we expect it to appear by making a PCR)



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