User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/14/2010/06/15
From OpenWetWare
< User:Nelson Augusto Berrocal | Notebook | WiFi Coli 2010 Wet Lab | 2010 | 06 | 14 | 2010 | 06
Project name | Main project page Previous entry Next entry |
June 15th, 2010While looking the items we received from Edimburgh we found that some primers, those for luxCDE, reverse and forward, were supposedly for LuxCDE from Photorhabdus luminescence but actually the primers were designed for LuxCDE from Photobacterium phosphoreum.
Then I made a PCR to get LuxAB from genomic DNA of Vibrio fischeri MJ11 following the next procedure:
|