User:Niepelm/Notebook/ICBP45/2010/09/23

ICPB45

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MCF7 PNNL-15cm all cells are dead and floating plated in Corning 15cm dishes 2 of the same cells in the same dishes in Mirra's incubator look better but still sick 2 of the same cells in BD Falcon dishes in Mirra's incubator look just fine --> problems with the dishes --> problems with the incubator? Order a new stack of incubators dedicated to this project. MCF7 RPLA-96well cells are attached but don't look great don't use because of problems with the same cell line HCC-70 SS-15cm some cells are attached but many float and form strange large clumps don't use HCC-1569/HCC-1954 density treated by Lili HCC-1187 CNI-96well scanned by Lili HCC-1954 CNI-96well added primary Ab o/n by Lili HCC-38 plating controls plate same cells into corning and BD Falcon dishes grow in three different incubators asses growth in the morning test for differences in plates and incubators