User:Omar Choudary/Notebook/Omar Choudary CHEM-571 AU-2011/2012/2011/08/31

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Contents

Objective

  • Synthesize Gold Nanoparticles(AuNP's) using Bovine Serum Albumin (BSA) and Chloroauric Acid (HAuCl4) in water.

Description

  • Created 10mL aqueous mixture of 0.0015 mM BSA and 0.25 mM HAuCl4 using water at room temperature.
  • Placed mixture into oven set to 80 degrees Celsius.
  • Using UV-Vis Spectrophotometer obtain UV-visible spectra (200 - 900nm) of mixture every 30 minutes for 2 hours.

Data

  • Theoretical Measurements:

1.5*10^-6 moles BSA / 1 L * .01 L * 66,776 g BSA / 1 Mole BSA = .001 g BSA

2.5*10^-5 moles HAuCl4 / 1 L * .01 L * 339.785 g HAuCl4 / 1 Mole HAuCl4 = 8.49*10^-5 g HAuCl4

  • Actual Measurements:

.001 g BSA

.006 g HAuCl4


  • Entire UV-Vis spectrum of mixture.

Image:OC8-31-1.jpg



  • UV-Vis spectrum focused at 550 nm to observe change in absorbance.

Image:OC8-31-2.jpg



  • Change in absorbance of mixture only at 550 nm. The increasing trend-line indicates AuNP synthesis is occurring.

Image:OC8-31-3.jpg

Notes

  • The molar ratio of Au/BSA was 1176.
  • The mixture was a clear solution.
  • The mixture was wrapped in aluminum foil after being removed from the oven.
  • Distilled water was used as the buffer in this experiment. A blank using just the distilled water was measured first and then BSA/HAuCl4 mixture was initially measured and then placed into the oven. The data projected on both graphs had been corrected using the blank.
  • All UV-Vis spectra were taken using a Shimadzu UV-2550.
  • The quartz cuvette used to obtain UV-Vis spectra had purple staining that could not be removed by washing.
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