User:Omar Choudary/Notebook/Omar Choudary CHEM-571 AU-2011/2012/2011/11/09

Chem-571

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Objective Perform gold nanoparticle(AuNP) synthesis to obtain purple homogeneous solution using 15.5 μM Bovine Serum Albumin (BSA), 2.9 mM Chloroauric Acid (HAuCl4), H2O, and Tris. Description Prepared four tubes: Tube 1: 0.1 mL BSA, 0.1 mL HAuCl4, 0.8 mL H2O. Tube 2: 0.1 mL BSA, 0.1 mL HAuCl4, 0.8 mL Tris Buffer (pH 9.36). Tube 3: 0.1 mL BSA, 0.05 mL HAuCl4, 0.85 mL H2O. Tube 4: 0.1 mL BSA, 0.05 mL HAuCl4, 0.85 mL Tris Buffer (pH 9.36). Tubes placed in heat block set at 80 degrees celsius, for 2 hours. Heat block was turned off after 2 hours to facilitate gradual decrease in temperature. Data Tube 1: Purple protein fiber aggregation, Molar ratio of Au/BSA = 187 Tube 2: Clear solution, Molar ratio of Au/BSA = 187 Tube 3: Purple solution (similar to experiment performed on 9/28), Molar ratio of Au/BSA = 98.4 Tube 4: Clear solution, Molar ratio of Au/BSA = 98.4 Notes