User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/29

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Cre(P1)

  • Host: Escherichia coli
  • Classification: tyrosine recombinase
  • structure
    • Length
    • Amino-Acid-Sequence
    • Domains
      • six tyrosine kinase conserved residues: R173, K201, R292, H289, W305, Y324 (nucleophilic tyrosine and catalytic pentad)
  • Target sequence: two identical simple sequence < 50 bp
  • Mechanism:
  • Engineering
    • Mutation
      • Mutation could change specificity to HK022's site
    • Chimeric
    • Directed-Evolution
  • Exision:
    • Unnatural-Host



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