User:Patrick A. Alayon
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Last Name
Alayon
First Name
Patrick
Preffered name
Patrick
Course/minor
20-Biological Engineering
Graduation Year
2009
Phone #
palayon AT mit DOT edu
Have you taken
7.05/5.07 (Biochemistry)
Taking this term
7.06 (Cell Biology)
No
7.02 (General Biology Lab)
No
5.310 (General Chemistry Lab)
No
Do you have any experience culturing cells (mammalian, yeast or microbial)?
No
Do you have any experience in molecular biology (electrophoresis, PCR, etc)?
No
Previous Lab Experience
High School Advanced Chemistry Lab experience
Genome Engineering Ideas
| M13 Genome Engineering Ideas | Ease of Modification in terms of overlap (1-4, 4 being hardest) | Ease of Modification relative to protein importance (1-4, 4 being hardest) | GFP Attachment |
|---|---|---|---|
| G1 | 3, 22 bp are shared with genes 4,11 | 2, pore gene | OK Target because pore will light up |
| G2 | 3, about 330 bp overlap with gene 11 | 4, DNA cutting gene | |
| G3 | 1, no overlap with any genes | 3, Entry gene | Good Target because we will see entry point of phage into cell |
| G4 | 3, 22 bp overlap with genes 1 and 11 | 2, pore gene | OK Target because pore will light up |
| G5 | 1, No overlap | 4, protects ssDNA | Good Target because we will see all phage DNA it surrounds |
| G6 | 1, No overlap | 1, Less important tail protein | OK Target because phage will light up inside cell, but few porteins to attach GFP to (5) |
| G7 | 2, 4 bp overlap with gene 9 | 2, Minor Head protein, starts secretion | OK Target because phage will light up inside cell, but few porteins to attach GFP to (5) |
| G8 | 2, 4 bp overlap with gene 9 | 3, Covers outside of phage | Good target because if successful, phage will light up outside cell. |
| G9 | 2, 4 bp overlap with genes 7,8 | 2, Minor Head protein, starts secretion | OK Target because phage will light up inside cell, but few porteins to attach GFP to (5) |
| G10 | 4, full overlap with gene 2 | 4, DNA Replication | OK Target if we want to see replication process |
| G11 | 4, full overlap with gene 1, partial overlap with gene 4 | 2, pore gene | OK Target because pore will light up |
