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The samples made in the previous lab period were flawed. Dr. Hartings remade the samples following these steps: (Link says unwritten).
Stock Solutions Used
Detailed versions of the protocol for UV-Vis can be found in earlier notebooks. Samples were prepared with an identical concentration of gold. The pH of the solutions were fixed to be 4,5,6,7,8,9,10,11,12 using 1 mM HCL. The solutions were placed in a cuvette and run through the fluorimeter.
This graph shows the absorbance of Fructose conc. 0.125 mM, pH 4-12 across 200-800 nm.
This graph shows the absorbance of Fructose conc. 0.25 mM, pH 4-12 across 200-800 nm.
This graph shows the absorbance at peak wavelength 530nm for Fructose concentration 0.125 mM (pH 4-12).
This graph shows the absorbance at peak wavelength 530nm for Fructose concentration 0.25 mM (pH 4-12).
Similar to the UV-Vis trial the group previously conducted, the samples of pH 11 and 12 (for both concentrations of fructose) had significantly lower absorbance than that of the samples with pHs 4-10. Fructose 0.25 with pH 11 and 12 had a very faint pink coloration to them. Samples with pH 4-10 all had similar absorbance across the spectrum. Max absorbance at 530 nm was also similar.
0.125 mM Fructose, 0.25 mM Au samples of pH 4-12.
0.25 mM Fructose, 0.25 mM Au samples of pH 4-12.