User:Patrick Hampson/Notebook/chem471/2016/09/21

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UV-Vis 2016921

Solution Preparation

Stock Solutions:

  1. 14.8 mM Fructose
  2. 49.1 mM BSA
  3. 3.73 mM Au
  4. 1mM HCL
  5. 1mM NaOH
  6. 1M NaOH
0.75 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water
4 500 0 0 335 253 316 3596
5 50 0 0 335 253 316 4046
6 5 0 0 335 253 316 4091
7 0 0 0 335 253 316 4096
8 0 5 0 335 253 316 4091
9 0 50 0 335 253 316 4046
10 0 500 0 335 253 316 3596
11 0 0 5 335 253 316 4091
12 0 0 50 335 253 316 4046


1.25 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water
4 500 0 0 335 422 316 3427
5 50 0 0 335 422 316 3877
6 5 0 0 335 422 316 3922
7 0 0 0 335 422 316 3927
8 0 5 0 335 422 316 3922
9 0 50 0 335 422 316 3877
10 0 500 0 335 422 316 3427
11 0 0 5 335 422 316 3922
12 0 0 50 335 422 316 3877

After samples were prepared, they were placed in the thermooven at 80 degrees Celsius for four hours.

Data

Image:160921 UVVIS 0.75.PNG

The graph above displays the absorbance of 0.75 mM Fructose samples of pH 4-12 across the 200 nm to 800 nm range.


Image:160921 A530 0.75.PNG

The chart above shows the absorbance of 0.75 mM Fructose samples of pH4-12 at 530 nm.


Image:160921 UVVIS 1.25.PNG

The graph above displays the absorbance of 1.25 mM Fructose samples of pH 4-12 across the 200 nm to 800 nm range.


Image:160921 A530 1.25.PNG The chart above shows the absorbance of 1.25 mM Fructose samples of pH4-12 at 530 nm.

Observations

As previously seen, the samples of pH 11 and 12 were clear. As expected, they had significantly lower absorbance.



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