User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/03
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Qualitative Analysis of Protease Activity
Continue the qualitative analysis of protease activity on AuNP fibers. Pepsin, Trypsin, Chymotrypsin, and Proteinase K will be tested.
NB - Pepsin has an optimal pH of 1.5-2, so a pH 2.14, 5 mM Citrate, 0.2 M NaCl buffer was made for Pepsin use. That may affect the results.
The AuNP fibers were spun down at 300 RPM for 4 minutes. The supernatant was removed, and the prepared solutions were added on top of the solid fibers. The tubes were placed in a shaker at 37°C. They will be analyzed tomorrow.
Proteinase K, Trypsin, and Chymotrypsin all had visible color changes. Pepsin did not have a visible color change. UV-VIS analysis can be found on tomorrow's page.