User:Paul Rothenberg/Notebook/Pauls Notebook/2014/07/07
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Further Control Reactions
Ran two more control reactions today, in 1-propanol and THF. We also ran the Mb Phosphate in ethanol at 25°C over 2 hours in Ocean Optics.
10 mg/mL of Mb Phosphate and Citrate were made in each solvent, along with minimal hemin in 5 mL of solvent. UV-VIS was run to determine concentration. The dilutions were done as follows:
The OPD-Peroxide reactions were set up in 4 reactions per solvent. These were shaken over two hours, and UVVIS was taken of each reaction.
From the timed run, here's the graph:
Here are the charts from the control reactions:
The propanol will definitely need to be run over time to see the reaction progression.