User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/08/17
From OpenWetWare
UNAM Genomics Mexico 2011  Main project page Previous entry Next entry  
LB media preparation/ Calculation of oligo dilutionsABSTRACT
When the medium is solid I added 15 g/L of bacteriologic agar.
I used a formula that says C_{0}V_{0}=C_{F}V_{F} where C_{0} is initial concentration, V_{0} is initial volume, C_{F} is final concentration and V_{F} is final volume. We want a final concentration of 5pM/μL and a final volume of 500μL. So by knowing the initial concentration we can use this formula to find the volume of oligo and water that has to be mixed to obtain the desired concentration.
C_{0}= 341.02 pM/mL Clearing for V_{0} we obtain that he have to mix 7 μL of oligo and 493 of water.
