User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/09/21

UNAM Genomics Mexico 2011

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Parts assembly/ PCRs ABSTRACT PCRs of HydEF1 and HydEF2. Today I did 8 PCR reactions, 4 to amplify HydEF1 and another 4 to amplify HydEF2, using the following proportions: H2O 23μL Phusion GC Buffer 10 μL dATP 1 μL dGTP 1 μL dCTP 1 μL dTTP 1 μL Primer fwd 5 μL Primer rev 5 μL DNA template 1 μL DMSO 1.5 μL Phusion HF pol 0.5 μL Cycles Gel Lanes Gel 95°C 5min 98°C 10s 30Cy 60°C 30S 30Cy 72°C 2 min 30Cy 72°C 10min 4°C Lane1 Ladder Lane2 HydEF1 Lane3 HydEF1 Lane4 HydEF2 Lane5 HydEF2 Cycles Gel Lanes Gel 95°C 5min 98°C 10s 30Cy 62°C 30S 30Cy 72°C 2 min 30Cy 72°C 10min 4°C Lane1 Ladder Lane2 HydEF1 Lane3 HydEF1 Lane4 HydEF2 Lane5 HydEF2