User:Pauline Wonnenberg/Notebook/CHEM 471 Fall 2017/2017/08/30

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Objectives Remake AuNP Solutions Prepare Amino Acid Solutions Prepare AuNP using Amino Acid Solutions Measure UV & Fluorescence of AA Stock Solutions Description AuNP not formed during previous laboratory; trial 2 for solutions below: Power of ten error was found, see updated calculations below: Goal 2.5mM HAuCl4 into 50mL flask Calculations 0.05L x 2.5x10-3M = 1.25x10-4moles HAuCl4 = 339.785g/mol 339.785g/mol : 1.25x10-4moles = 0.04247g = 42.47mg of HAuCl4 Preparation of 3 different amino acid solutions with the same concentration as BSA (250 μM): Choice of amino acids Allopathic - Isoleucine; Aromatic - Phenylalanine; Amine - Arginine Isoleucine Calculations: 0.05L x 250x10-6M = 1.25x10-5moles Molar mass of Isoleucine: 131.18g/mol 131.18g/mol x 1.25x10-5M = 0.001639grams Phenylalanine Calculations: 0.05L x 250x10-6M = 1.25x10-5moles Molar mass of Phenylalanine: 165.19g/mol 165.19g/mol x 1.25x10-5M = 0.002065grams Arginine Calculations: 0.05L x 250x10-6M = 1.25x10-5moles Molar mass of Arginine: 174.20g/mol 174.20g/mol x 1.25x10-5M = 0.0021775grams Data Prepare AuNP using Amino Acid Solutions: Aromatic - Phe Ratio pH Values  20 3.327  80 3.273   160 3.244  Amine - Arg Ratio pH Values  20 3.372  80 3.260   160 3.272  Aliphatic - Iso Ratio pH Values  20 3.235  80 3.292  160 3.338  Measure UV of AA Stock solutions: Following the above table, here is the UV-Vis spectrometer collected analyzing a sample of the Phenylalanine stock. Following the above table, here is the UV-Vis spectrometer collected analyzing a sample of the Arginine stock. Following the above table, here is the UV-Vis spectrometer collected analyzing a sample of the Isoleucine stock.