User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/12
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make 100mL of solutions so..... 12.11grams of tris /100mL were mixed.
For further breakdown by run and concentration see attached spreadsheet
Link to spreadsheet for Tris buffer pH 10 UV-Vis.
A. The results were fairly consistent with there not being an increase in the concentration of the gold nano particles. For all 7 samples, the peak stayed at generally the same wavelength for all three tests and therefore the entire length of experimentation time. Thus it seems that changes in the pH of the tris buffer did not result in a noticeable change in abs. However, it is important to note the drop in absorbency for some of the concentration samples. For example, the 1x10-4 concentration shows the most obvious drop between The first time the were measured after the addition of tris (immediately) and two hours later when the third run of UV-Vis was taken. Whether or not this is simply a result of noise or actually because an increase in the amount of AuNP dissolved in the buffer solution is unknown because a blank of water was not measured during these tests.