User:Puja Mody/Notebook/Magnetite Shenanigans/2014/09/19

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Dye Addition

  • Preparation of Rhodamine 6G stock solution
Rhodamine 6G '
Molecular Weight (g/mol)479.02
mass(g)2.3951
volume stock (L)0.01
Concentration (M)0.5
Real concentration of Rhodamine used '
concentration (M)0.005
volume stock (L)0.1

Dye addition to samples was done at a range of concentrations from 20 μM - 200 μM and was added to 1mL of sample. The samples need at least 24 hours in order to soak in the dye.

  • Fluorescence of the water sample which the nanoparticles/ rhodamine will be analyzed to determine the total R6G concentration adsorbed by the hydrogel complexes.
Sample Number Internal Conc. Dye in each sample (M) volume of dye added in uL
10.000020.0000044
20.000040.0000088
30.000060.00001212
40.000080.00001616
50.00010.0000220
60.000150.0000330
70.00020.0000440

Samples were placed in eppendorf tubes and manually shaken/ stirred for approximately 2 minutes each in order to mix dye and prepare samples for absorption/ crosslinking. Samples were left overnight.

NOTES

    • From UV-Vis spectra collections, it was determined that the true concentration of the R6G that was used was: 398.7 uM


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