User:Puja Mody/Notebook/Magnetite Shenanigans/2014/09/24

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Magnetic Stimulus Testing General Protocol: Centrifuge the 1.5 mL eppendorf tubes with the samples for 15 minutes at 13000 rpm Remove the R6G supernatant and store in clean eppendorf tub Add 1 mL deionized H2O to the eppendorf tube. Remove the deionized water. Repeat this rinsing process a minimum of three times Add 1.3 mL of Water to the eppendorf tube Place samples on UV/Fluorescence light to see if dye is present in hydrogel samples Centrifuge the samples in the same manner as in step 1. Remove this deionized water and place in a clean eppendorf tube for later testing ( in order to test if dye is coming out with centrifuging) Add 1.5 mL of water to sample Vortex the sample for ~2 minutes (until the sample is in homogeneous suspension) Apply magnet to the outside of the eppendorf tube for a minimum of 30 seconds. (until all the sample has aggregated Vortex sample again to re-suspend the hydrogels (at least 15 seconds) Re-apply magnet Complete this vortex/magnet process for 4 minutes Analyze the water using fluorescence Analysis of the supernatant Place the supernatant in a clean cuvet and place into UV-Vis. Run UV-Vis analysis for 200-800nm. If absorbance is too high, dilute samples in order to determine concentration of R6G remaining.