User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/01/22

Drosophila melanogaster Genetics

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Spermatogenesis With gfp-myb, rfp-h2av samples: Heather performed the following yesterday (1/21/10): Incubate with primary antibody in 2% NGS O/N at 4C Anti-sa with 1:100 anti-sa from guinea pigs Anti-bam with 1:5 anti-bam from mouse Totpro with nothing, continued blocking Today: Wash 3 x 10 minutes in 1x PBS-T Incubate with secondary antibody in 2% NGS (1:500 of respective secondaries conjugated with 633 fluorophore) Except totpro, remained in 1x PBS-T 2hr at RT Wash 3 x 15 minutes in 1x PBS-T Keep over the weekend in PBS-T. Will stain totpro sample with totpro on Monday and mount the anti-bam and anti-sa samples.