User:Roberta Diaz Jimenez/Notebook/CHEM 471 Experimental Lab/2015/09/30

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Objective

Make standardized curves for determining peptide concentration using fluorescence analysis.

Procedure

We followed the protocol in Dr. Hartings' notebook. The following are detailed steps we took:

  • Protease Sample Prep.
  1. Used eppendorf tube no. 13 that weighed 1.02669g, and contained 0.00124g of trypsin.
  2. Added 1mL of phosphate buffer.
  3. Final concentration: 53.22┬ÁM
  • Sample Prep.
  1. In a 1mL eppendorf tube add:
    1. 0.9812mL of lysozyme solution
    2. 0.0188mL of trypsin solution
  • Bank Prep. (no lysozyme)
  1. In a 1mL eppendorf tube add:
    1. 0.9812mL of buffer solution
    2. 0.0188mL of trypsin solution

Data


Personal tools