User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/21

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Ligation

Reaction

1.67 μL pMAL-c2X Digest (50 ng)
1.33 μL PCR Product Digest (37 ng)
   7 μL MilliQ
  10 μL Quick Ligase Buffer
   1 μL Quick Ligase

Conditions

  • Incubate at room temperature for 1 min

Control

  • 1 sample replaces PCR Product Digest with MilliQ water.

Transformation

  • XL1-Blue Competent Cells
  • Plated onto 4 Amp plates (3 sample, 1 control)



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