User:Samiye Yaman/Notebook/Lab 571/2011/11/15

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Entry title

Objective

Synthesizing nanoparticles

Description

PART I -Label 5 epi tube with 100ul BSA, 50 ul HAuCl 850ul water in each of them. (Concentration of BSA is 30uM and HAuCl is 4.17uM.)

-Place all the epi tubes to heating block which is at 80 degree celcius.

-At the end of two hours, the temperature started to dropped slowly.

( 2-1 ): no addition of refolding buffer

( 2-2 ): quick transfer of refolding buffer

( 2-3 ): addition of refolding buffer drop by drop

( 2-4 ): quick transfer of refolding buffer at high temperature

( 2-5 ): addition of refolding buffer drop by drop at high temperature

-The refolding buffer is 1M Tris with a ph of 6.8. It is also placed in to the heat plate.

PART II

-1ml of BSA, 0.5 mL of HAuCl and 8.5 ml of water was placed in to two test tubes.

-The test tubes were placed in to the oven which was at 78degree celcius for two hours. (Concentration of BSA is 30uM and HAuCl is 4.17uM.)

Data

Part I

( 2-1 ): light purple color(most purple solution)
( 2-2 ): light purple color- after addition of refolding buffer become light pink
( 2-3 ): light purple color- after addition of refolding buffer become light pink
( 2-4 ): medium purple color- after addition of refolding buffer become light pink
( 2-5 ): medium purple color- after addition of refolding buffer become light pink


(adding slower, lighter in color)


Part II

Both test tubes had no fiber formation. Clear solutions stayed remain.

Notes

This area is for any observations or conclusions that you would like to note.


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