User:Samiye Yaman/Notebook/Lab 571/2012/03/06

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Objective

Determining the effect of the mole ratio of Au/BSA on AuNP synthesis

Description

Effect of Au/BSA Ratio

-Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4:

--90 Au/BSA

--110 Au/BSA

--130 Au/BSA

--150 Au/BSA

-Dilute to 1 mL with water.

-Place in oven at 80°C.

-Leave solutions in oven for 3.5 hr.

-Centrifuge solutions for 10 min at 13200 rpm.

-Take UV-vis data of supernatant.

Effect of Tris Buffer on Protein Fibers

-Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12.

-Take UV-vis absorption data.

-Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12.

Data

90 Au/BSA Ratio

Image:90_ratio.JPG

110 Au/BSA Ratio

Image:110_ratio.JPG

130 Au/BSA Ratio

Image:130_ratio.JPG

150 Au/BSA Ratio

Image:150_ratio.JPG

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