User:Samiye Yaman/Notebook/Lab 571/2012/03/06

Biomaterials Design Lab

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Objective Determining the effect of the mole ratio of Au/BSA on AuNP synthesis Description Effect of Au/BSA Ratio -Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4: --90 Au/BSA --110 Au/BSA --130 Au/BSA --150 Au/BSA -Dilute to 1 mL with water. -Place in oven at 80°C. -Leave solutions in oven for 3.5 hr. -Centrifuge solutions for 10 min at 13200 rpm. -Take UV-vis data of supernatant. Effect of Tris Buffer on Protein Fibers -Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12. -Take UV-vis absorption data. -Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12. Data 90 Au/BSA Ratio 110 Au/BSA Ratio 130 Au/BSA Ratio 150 Au/BSA Ratio