User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/11

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UV-VIS

Purpose

To observe the change in absorbance of AgNO3 NP's at pH 4-12 for fructose at concentrations 0mM and .0625mM

Procedure

  1. Solutions were prepared on 10/04/2016
    1. The solutions had been heated in an 80C oven for four hours and then sat at room temperature. UV-Vis spectra were taken of each sample from 200-800nm.
    2. The test tubes were kept completely wrapped in tin foil the entire time, because AgNO3 is light sensitive.
    3. All of the solutions were yellow and clear, except for 0mM Fructose pH4 and 6, which were yellow and opaque, and pH 9, which was clear with precipitate formed at the bottom of the test tube, these solutions are shown below.

AgNO3 0mM Fructose pH4-6 (left to right)

AgNO3 0mM Fructose pH 9

Data

  1. Graphs for 0mM Fructose

Absorbance v. Wavelength

Absorbance at 407nm

  1. Graphs for 0.0625mM Fructose

Absorbance v. Wavelength

Absorbance at 407nm


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