UV-VIS
Purpose
To observe the change in absorbance of AgNO3 NP's at pH 4-12 for fructose at concentrations 0.75mM and 1.25mM
Procedure
- Solutions were prepared on 10/26/2016
- The solutions had been heated in an 80C oven for four hours and then sat at room temperature. UV-Vis spectra were taken of each sample from 200-800nm.
- The test tubes were kept completely wrapped in tin foil the entire time, because AgNO3 is light sensitive.
Solutions
- All solutions at pH 4-10 for both concentrations of fructose, 0.75mM and 1.25mM were a pale yellow color with some cloudiness.
- Solutions at pH 11 and 12 for both concentrations were a bright orange color with some amount of cloudiness
- Below are the samples at pH 11 and 12 from all six concentrations of fructose (0mM, 0.0625mM, 0.125mM, 0.25mM, 0.75mM and 1.25mM)
Data
- Graphs for 0.75mM Fructose
Absorbance vs Wavelength
Absorbance at 400nm
- Graphs for 1.25mM Fructose
Absorbance vs Wavelength
Absorbance at 400nm
|