User:Sara K. Mattson/Notebook/Chemistry 671/2016/11/01

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UV-VIS Purpose To observe the change in absorbance of AgNO3 NP's at pH 4-12 for fructose at concentrations 0.75mM and 1.25mM Procedure Solutions were prepared on 10/26/2016 The solutions had been heated in an 80C oven for four hours and then sat at room temperature. UV-Vis spectra were taken of each sample from 200-800nm. The test tubes were kept completely wrapped in tin foil the entire time, because AgNO3 is light sensitive. Solutions All solutions at pH 4-10 for both concentrations of fructose, 0.75mM and 1.25mM were a pale yellow color with some cloudiness. Solutions at pH 11 and 12 for both concentrations were a bright orange color with some amount of cloudiness Below are the samples at pH 11 and 12 from all six concentrations of fructose (0mM, 0.0625mM, 0.125mM, 0.25mM, 0.75mM and 1.25mM) Data Graphs for 0.75mM Fructose Absorbance vs Wavelength Absorbance at 400nm Graphs for 1.25mM Fructose Absorbance vs Wavelength Absorbance at 400nm