User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/10/05

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Ocean Optics Round 4

pH 7

Volumes and concentrations: Image:Screenshot 2016-10-05 at 12.50.46 PM.png

We accidentally set the temperature to room temperature instead to 80 degrees. No increase in absorption occurred even after an hour and a half, so almost no nano particles were formed. Have to re-run pH 6 Ocean Optics.


Data Analysis

Image:Oceanoptics161006pH7BSA.PNG

The above chart shows the UV-Vis maximum absorbance over time done on the Ocean Optics instrument of pH 7 Au NP with BSA=3.125 uM. The chart shows that pH 7 formed many fewer NPs than other pH solutions have in the past.


Image:Oceanoptics161006pH7BSAA530.PNG

The above chart shows the UV-Vis A(535.15) over time done on the Ocean Optics instrument of pH 7 Au NP with BSA=3.125 uM. The chart shows that pH 7 formed many fewer NPs than other pH solutions have in the past, but there is still a trend showing an increase over time.


Image:Oceanoptics161006pH7BSAWAVELENGTH.PNG

The chart above shows an increase in the maximum wavelength around 7000 sec. The readings making up this chart are much "shakier" than previous runs.

Lysozyme presentation

  • absorbance jump at 30 min

pH 5: shifted to left. dip in absorbance. pH 6 absorbance lower. start doing: change from time 0. drop them together. absorption of single wavelength vs time. at 30 minutes ~ 560 nm you see jump. absorbing at longer wavelengths . stability of protein at low pH.

integrated intensity: increases , decreases , increases. emission shifting to right with time. monitoring a specific wavelength. compare to integrated intensity which has a shifting maximum. overlap kinetics with absorbance. have to compare max intensity for absorbance : allowing fluorescence shift but not UV shift. max absorbance. many chemical processes. fluorescence dip: time at which making nanoparticles (35 min) no absorption features stand out before, but emission stands out . protein - gold interaction . at 5 min: broader fluorescence. luminescent gold complexes. unfolded within first 5 minutes. add graph for just protein. start thinking of deconvolute fluorescence in diff states. one wavelength is increasing . large peak at 380 -- overlap. component analysis. software ? areas under curve vs time. fit to 4 - 5 Gaussians.



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