User:Shahar Sukenik/Notebook/p53CT expression in minimal media/2010/08/03

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03/08/10: Bacteria growth

  • Started growing at 10:00. Had 2 x 250 mL M9 media (one for 17°C and one for 37°C) and 1 50 mL 2xYT+NPS as control.
  • Inserted 1:100 volume of starter solution, which seemed good.
  • Started checking OD:
The table's caption
Time 17°C 37°C ctrl/37°C
10:00 0.092 0.096 0.094
16:00 0.100 0.09 1.14
18:40 0.100 0.08 -
21:10 0.098 0.096 -
10:10 (4/8/10) 0.100 0.1 -
  • At 16:00 I took the ctrl flask from 37°C, added 6 μL 840 mM IPTG and inserted to 17°C to induce expression O/N.
  • M9 flasks did not show any growth.



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