User:Spenser Skates/Notebook/pTKX luciferase biobrick/2008/04/28

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Incorporation of PCR BB product into backbone

Cut PCR results and Kan backbone seperately with EcoRI and PstI, then denatured restriction enzymes. Ligated DNA together with T4 DNA ligase.
Transformed ligated product into E.coli by heatshock
Plated transformants on amp+kan plate.

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