User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/09

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BSA and HAuCl4 Solutions Au solution of HAuCl4:3H2O MW = 393.83 g/mol in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O actual amount used: 10.01mg therefore, I need 11.0048mL H2O 89-98,000 BSA solution MW = 66776g in 20mL 15μM solution, I need 20.032mg BSA actual amount used: 20.120mg BSA therefore, I need 20.0879mL of H2O Gold:BSA ratios Ratio 1: 60 Au:BSA fixed: .25mM Au new BSA: 0.00417mM solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O Ratio 2: 90 Au:BSA fixed: .25mM Au new BSA: 0.00278mM solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O Ratio 3: 120 Au:BSA fixed: .25mM Au new BSA: 0.00208mM solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O Ratio 4: 150 Au:BSA fixed: .25mM Au new BSA: 0.00167mM solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O All solutions put into the 80°C oven at 12:07PM and taken out at 4PM