User:Steven J. Koch/Notebook/Kochlab/2009/09/15/EarI pBR322
Steve Koch 20:29, 15 September 2009 (EDT): Using restriction cross-correlate software & pBR322 sequence from genbank (http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/GenBank/pBR322.gbk.txt), I find two EarI sites:
Thus, should produce two fragments, one approx 2556; the other approx 1804 bp. From the top strand, the overhangs read:
- GCT (This is the overhang we have on our adapter duplex)
If we gel extract the big piece (approx 2556), it will be complementary to a 5' GCT overhang. So, I think we are all good. This is consistent I think with my earlier work (see below).