User:Steven Moss/Notebook/AU CHEM-570

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Project Description/Abstract

The overall goal of our project in the Biomaterials Design Lab, is to create a novel system for the functionalization of gold nanoparticles. The individual project goals for this semester in order to reach that point, are as follows:

  1. Expressing and purifying both Maltose Binding Protein (MBP) with attached intein and Green Fluorescent Protein (GFP) which has been previously mutated and has an attached His-tag
  2. Testing intein chemistry on a gold nanoparticle with MBP-intein and GFP (intein=Mxe Gyr A)
  3. Functionalizing a gold nanoparticle with GFP

Notes

  • Some of the laboratory procedures are stored in Tamra or Catherine's openwetware biomaterials design notebooks.


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