User:Stuart McKellar/Notebook/Bird Sex Testing/2012/11/23
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Yesterday failed to yield a single successful result. The two steps that were universal across all samples were number of cycles, an extended 72C period of 20 mins after 25 cycles, and 0.5U taq.
Aim: Today I will try with 3 different levels of taq and dna and run everything else the same as for the initial successful run. The tubes contents will be as follows:
DNA From R5#9 (DNA 186.53 ng/ul)
Then as follows for each tube:
All of the tubes had 24.5 ul total. They had 21ul of MM, instead of 21.5.