User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/02
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Trying all loris with 65°C PCR - p2/p8Aim: To see if at 65°C i can differentiate males from females. Method: Master mix for all 8 samples plus -ve control(10 units of MM):
Tubes labelled as follows:
Add 1ul of relevant DNA to each tube and 24ul of MM. PCR: 94°C for 2m30s, 35 cycles of: 95°C 30s, 65°C for 30s, 72°C for 30s; final elongation 72°C for 5 mins( actually went 10 mins by accident). results to be determined via agar gel electrophoresis on 1% gel stained using gelgreen.
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