User:Susan Schultz/Notebook/Experimental Biological Chemistry/2011/09/20

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Objective

Extract Maltose Binding Protein from ER2566 E.coli with the pMAL-pIII plasmid.

Description

See in pMal protein fusion and purification system manual. <http://www.neb.com/nebecomm/ManualFiles/manualE8000.pdf>

The frozen cells in column buffer from the growth cultures prepared on 9/14/11 were thawed in a water bath. They were then sonicated in 15 second intervals for a total of 90 seconds, and were kept in ice between sonications. The mixtures were then centifuges for 30 minutes at 9000g. The supernatant, containing the protein was removed and purified using a column.

Amylose Resin was poured into a 15mL column and it was washed with 120mL of column buffer. This column prep was run overnight.

Data

  • Add data and results here...

Notes

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