User:Susan Schultz/Notebook/Experimental Biological Chemistry/2011/10/26

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Objective

In Progress

De-stain SDS-PAGE protein gel.

AuNP Synthesis

Description

AuNP Synthesis

Repeat AuNP snythsis reaction in water and run a control reaction of just BSA in water

Reaction 1- 1ml 15μM BSA + 2ml 0.75mM HAuCl4 removing from heat for 10 minutes every 30 minutes

Reaction 2- 1mL 15μM BSA removing from heat for 10 minutes every 30 minutes

De-Staining

Remove Coomassie Brilliant Blue R250 from gel

Add de-staining place on shaker

When de-staining solution has become saturated, remove and replace

Leave of shaker overnight

Data

BSA and Water Only- No Observed Change

AuNP Synthesis- 1st observed gold colored fluid aggregating near the top of the solution, then black fibers apeeared and balled together.


Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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