User:Susan Schultz/Notebook/Experimental Biological Chemistry/2011/10/26

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In Progress

De-stain SDS-PAGE protein gel.

AuNP Synthesis


AuNP Synthesis

Repeat AuNP snythsis reaction in water and run a control reaction of just BSA in water

Reaction 1- 1ml 15μM BSA + 2ml 0.75mM HAuCl4 removing from heat for 10 minutes every 30 minutes

Reaction 2- 1mL 15μM BSA removing from heat for 10 minutes every 30 minutes


Remove Coomassie Brilliant Blue R250 from gel

Add de-staining place on shaker

When de-staining solution has become saturated, remove and replace

Leave of shaker overnight


BSA and Water Only- No Observed Change

AuNP Synthesis- 1st observed gold colored fluid aggregating near the top of the solution, then black fibers apeeared and balled together.


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