User:Susan Schultz/Notebook/Experimental Biological Chemistry/2011/11/09

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Objective Run AuNP synthesis reactions with different Au/BSA ratios and concentration to investigate the conditions of fiber formation. Description The following reactions were run: Place three test tubes in a water bath at 80°C test tube 1: 50μL BSA + 50μL HAuCl4 + 900μL H2O test tube 2: 50μL HAuCl4 + 100μL BSA + 850μL H2O test tube 3: 80μL BSA + 80μL HAuCl4 + 640μL H2O After two hours let water bath cool overnight Place three test tubes in heat block at 80°C test tube 1: 50μL BSA + 50μL HAuCl4 + 900μL H2O test tube 2: 50μL HAuCl4 + 100μL BSA + 850μL H2O test tube 3: 80μL BSA + 80μL HAuCl4 + 640μL H2O Place test tube (80μL BSA + 80μL HAuCl4 + 640μL H2O) in thermocycler run at 80°C for two hours then decrease 2°C every 2 min until the sample reaches room temperature Run microwave reaction with 400μL BSA + 400μL HAuCl4 and water to the top (about 8 mL) at 300 W at 80°C for 30 min Data Figure 1. 80μL BSA + 80μL HAuCl4 + 640μL H2O) in thermocycler Figure 2. 80μL BSA + 80μL HAuCl4 + 640μL H2O in heating block Figure 3. 50μL BSA + 50μL HAuCl4 + 900μL H2O in heating block Figure 4. 50μL HAuCl4 + 100μL BSA + 850μL H2O in heating block Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.