User:Susan Schultz/Notebook/Experimental Biological Chemistry/2011/11/30
|Au/BSA ratio gradient|| Main project page|
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Determine the Au/Gold ratio at which the solution becomes fibers
Created a gradient with increasing AU/BSA ratios.
Gold stock- 2.9mM BSA stock 17.7μM
Up to this point we have found that at low Au/BSA ratios a solution is formed, and at high Au/BSA ratios fibers are formed. Today we are running a gradient to determine where and how that change occurs.
Left in heatblock at 80°C for approximately 3 hours.
When the tubes were removed from the heatblock, the test tube 1 and 2 initially appeared to be a purple solution. Within a minute purple fibers came out of solution and the solution became clear. The other test tubes appeared as shown below. There was a small golden aggregation in test tubes 3-6.
This area is for any observations or conclusions that you would like to note.