User:Sydney Marshall/Notebook/Protease Project/2015/10/20

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Objective Perform OceanOptics on 10 um Proteinase K Description For full protocol, see here. Preparing AuNP fiber samples Five 1 mL predried fiber samples were obtained 0.2mL of 100mM Tris and 50mM CaCl2 pH 8 buffer was added into the first eppendorf tube of fiber samples This was done for all five samples All five samples were combined into the cuvette to run with the ocean optics Preparing Protinase K solution Proteinase K tube 1G was mixed with 1 mL of 100mM Tris/50mM CaCl2 pH 8 buffer Proteinase K conentration: (0.00116g)*(1mol/28,900g)*(1/0.001L)= 0.0000553 M Proteinase K Amount of Proteinase K solution needed for 3mL with 1μM concentration: M1*V1 = M2*V2 => (55.3 μM)*(V1) = (1 μM)*(3 mL) => V1 = 54 μL Proteinase K Amount of Buffer solution need to get to 3mL: (3mL total)-(0.054 mL Protinase K solution) - 1mL fibers = 1.946 mL buffer Proteinase K, AuNP fiber samples, and buffer was mixed in a cuvette and run through the OceanOptics. Results INSERT GRAPHS HERE