User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/09/19

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Sept 19 - Film Absorbance of Arsenic and Mercury

  • Group Members Andrew Farag Sr (Undergrad) and Michael Bible Jr (Undergrad)
  • Group Name : AMT


  1. Finish analyzing PVA-malachite green data
  2. Start analysis on X-ray
  3. Start making new PVA and PVA clay films
  4. Set up experiments (like the malachite green experiments) to see how well the films absorb arsenic and mercury
    1. Notes: both of these are toxic metals, and we need to understand how to use/work with them.
      1. You MUST read the following before starting in lab today
      2. Information sheet on As
      3. Information sheet on Hg
      4. Note: Both metals are volatile. You MUST only work on solutions of these in the hood. If you take solutions of these out of the hood, they must be in sealed containers. Also, any container that has As or Hg in it must be rinsed (and the rinse must go into the waste containers).

(Objective from Dr. Hartings lab notebook Dr. Hartings)


For your Hg and As experiments, you need to have three concentrations: 150ppm, 100ppm, and 50ppm.

Notes and Calculations

Mercury stock solution

  1. 0.0908g HgCl2
    1. (0.0908g HgCl2)*(1mol HgCl2/271.5g HgCl2)*(1mol HgCl2/1mol Hg)*(200.59g Hg/1mol Hg) = 0.0671g Hg
    2. put in 250mL volumetric
    3. 268ppm Hg

Table of Solution Preparation The stock solution for Arsenic was prepared by Eleni.

As Stock Solution 0.0499M
As stock solution 3738.61ppm
As 150ppm solution 4mL stock in 100mL
As 100ppm Solution 2.76mL stock in 100mL
As 50ppm solution1.32mL stock in 100mL

The mercury solutions were made by Michael Bible

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