User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/09/24

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Sept 24 - AA analysis of As samples

  • Group Members Andrew Farag Sr (Undergrad) and Michael Bible Jr (Undergrad)
  • Group Name : AMT

Objective

  1. Continue processing films from last week
  2. Make two new films
    1. One containing the exchanged clay that your group made
    2. One containing the exchanged clay that another group made
  3. Run AA on the arsenic samples
    1. Make As standards (Note: AA will consume around 5mL of sample)
      1. 0ppm
      2. 10ppm
      3. 20ppm
      4. 30ppm
      5. 40ppm
      6. 50 ppm
    2. Dilute your samples so that their original concentration falls somewhere in this range.
    3. Make calibration curve and determine the concentration of As using AA.
Objective from Dr. Hartings lab notebook Dr. Hartings' Notebook

The original objective were modified, because the Arsenic lamp for the AA was burnt out. The new goal for the class was to set up an experiment with the PVA and PVA with clay films using Rhodamine 6G. This experiment will determine how well the films can filter out fluorescent contaminations from water. The stock solution of Rhodamine 6G was at 159 micromoles. The concentrations for the standards are 1, 10, and 20 micromoles.

Notes on the start of the synthesis of films with cation exchanged clay

Today we made films using the cation exchange clays begun on September 10. One of the films was made using the tributylhexadecylphosphonium bromide that our group started on 9/10 and the other was made using (1-hexadecyl)triphenylphosphonium bromide that Eleni's group started synthesizing on 9/10.

Our cation exchanged clays were made as follows:

  • PVA + tributylhexadecylphosphonium bromide exchanged clay
    • Mass of PVA = 1.0025g
    • Mass of tributylhexadecylphosphonium bromide = .1100g
    • Volume of Water = 7.0 mL
  • PVA + (1-hexadecyl)triphenylphosphonium bromide exchanged clay
    • Mass of PVA = 0.9995g
    • Mass of (1-hexadecyl)triphenylphosphonium bromide = 0.1103g
    • Volume of water = 7.2 mL

R6G Experiment

  • Stock solution was 159uM
  • The three concentrations of R6G were created from a stock solution of 159 μM R6G.
    • 1μM R6G - 0.629 mL of stock into 100mL
    • 10μM R6G - 6.289 mL of stock into 100mL
    • 20μM R6G - 12.578mL of stock into 100mL

The solutions were prepared by Eleni Kalivas

Image:Massesoffilm.png

Experiments were also set up using the three concentrations of RG6 with just glass vials, to determine if any fluorescents is absorbed into the glass.

  • Group BEM set up experiments for 1 and 10 μM R6G
  • Group MJJ set up experiments for 10 and 20 μM R6G
  • My group (AMT) set up experiments for 1 and 20 μM R6G


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