User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/11/05

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Nov 5- Ribbons

Objective

  • Using ribbons run a 20 ppm MG timed experiment, run XRAY and DSC
  • Make beads

Notes

Michael represented AMT for learning a synthesis to make PVA with clay beads, the following notes are from his notebook

  • Start bead synthesis with Mary and Melvin.

Synthesis of PVA-clay Beads

  • 0.51520 g PVA + 0.05154 g NaMT in 6 mL 10% HCl.
  • 750 uL of 0.8% glutaraldehyde was added to 20 mL of ethyl acetate. The mixture was stirred at maximum speed on a stirrer.
  • The PVA-clay-HCl solution was added to the ethyl acetate mixture in a drop wise manner.
  • 750 uL of 0.8% glutaraldehyde was added to 20 mL of ethyl acetate. The mixture was stirred at maximum speed on a stirrer.
  • Depending on the conditions, microbeads should form between 2-3 minutes. As soon as the beads are visible, IMMEDIATELY ADD 25 mL of 0.2 M NaHCO3. Stir for at least 4 minutes, 15 minutes at most.
  • Beads will be ready once a clear separation of the water and organic phase is observed. Beads will precipitate to the bottom of the beaker. There will still be some beads left in the organic solvent phase.
  • Pour the contents into the falcon tube.
  • Centrifuge for 1 min at 3000 rpm.
  • Afterwards, remove the organic phase and suspend the beads in distilled water.

Protocol adopted from Mary.

  • Andrew and myself worked with ribbons synthesized by Becky, Melvin, and Eleni

Ribbons

  • The weight of the ribbons used was 0.2875g
  • Class stock ws made by Eleni Kalivas, and Becky Rodriguez
  • Water background measuremens and the 20ppm MG made from 230ppm stock MG measurements were taken by Madeleine Bee.

Figure 1. UV-Vis Analysis of timed experiment on 0.2875g of Ribbons in 20 ppm of MG. Image:ribbions.jpg


  • AMT was spot #24 on the DSC and spot #4 for X-ray. The mass of ribbons used on the PXRD plate was 0.1266g. The mass of ribbons used in the DSC cup was 0.0048g.


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