User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/11/07

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Nov 7 - Class Paper

Objective

  • Review X-Ray data
  • Synthesize beads
  • Review Class Paper information

Notes

Michael continued to represent AMT with bead synthesis. The following are notes fromhis notebook

Synthesize beads with Mary and Melvin.

Description

  • Stir 30 mL of ethyl acetate + 500 uL of glutaraldehyde
  • Add your PVA-clay (approximately .5 g of PVA and .0575 g NaMT) in a dropwise manner. Immediately start timing the reaction for 2 minutes when you do the first drop.
  • After 2 minutes, slowly add 7 mL of 0.2 M NaHCO3.
  • Once completed, transfer to a falcon tube and centrifuge for 1 minute at 3000 rpm, rotor 41.
  • When beads are separated from the organic phase, pipet out the organic phase and re-suspend the beads in distilled water.

Protocol adopted from Mary.

X-Ray XRD results were saved using the following instructions

  • PDXL- click OK
  • Load data
  • Export
  • Use first two columns to graph
  • In the graphs the 1st peak would be any sodium, 2nd peak Tape and PVA, 3rd Peak Pan

Class Paper Measurements

  • mg analyte/mass polymer
  • Kinetics
    • Beads, PVA, PVA with clay
    • Films
    • Other Absorbents like activated carbon and zeolite (this makes the experiments relevant)
  • Accurately
    • stocks always the same across the groups, measure each day and use calibration curves averages
  • Mass
    • dehydrate the sample
  • Other measurements
    • SEM
    • BET
    • Bead size
    • DSC
    • XRAY



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