User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/11/26

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SEM and EDS of PVA+Clay Beads

  • Group Members Andrew Farag Sr (Undergrad) and Michael Bible Jr (Undergrad)
  • Group Name : AMT

Objective

To discover the morphology and elemental characteristics of the PVA+clay beads by using an Scanning Electron Microscope (SEM) and Engery Dispersive Spectrometry (EDS)

Notes

The beads were first analyzed using an Olympus SZX7 steromicroscope with a Highlight 3100 attachment, to determine the geranal size, shape, and color of the beads. This step is important because the SEM cannot determine color differences.

  • Image 1, Olympus SZX7

Image:stero2.jpg

  • Image 2, the general overview taken on the steromicroscope are pictured.

Image:Image 2.jpg

  • Next a stub was set up with the three different types of beads present, white, clear and a mixture. An image of the stub was taken with the knotch faces down so that the orientation of the beads could be determined in the SEM.
  • Image 3, the stub set up with clear, white, and mixed beads.

Image:Image 3 .jpg

  • The beads were analyzed on a Jeol JSM-661OLV Scanning Electrom Microscope with low vacuum.
  • Image 4, stub and holder before inserted into SEM

Image:stub and holder2.jpg

  • Image 5, Jeol SEM

Image:SEM2.jpg

  • Image6, Inside of SEM

Image:inside SEM2.jpg

  • Image 7,white beads

Image:White beads.jpg

  • Image 8, clear beads

Image:Clear beads.jpg

  • Image 9, mixed beads

Image:Mixed.jpg

Overall the mixed and the white beads have a much smoother surface than that of the clear beads. It was my theory that the clear beads did not have as much clay in them as the white and the mixed. EDS was completed on the three types of beads to confirm that theory.

  • Image 10, EDS results

Image:EDS.jpg

  • The EDS results confirmed that the clear beads had half the amount of sodium then the white and mixed beads.
  • EDS also confirmed that the bright white particles on the surface of the beads, were salt deposits.


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