Feb 18- Growth of cells
Objectives
- To place films from 02/13/2015 XRD molds into oven to cure
- To prepare a new batch of TiO2/epoxy and epoxy alone coatings
- Thaw NaaBlue Cells
- Grow cells
Growth of cells
- NaaBlue Cells were taken from the -20 degrees Celsius freezer and thawed on ice for one hour
- Used all sterile supplies, (round bottom tubes and pipets) and ethanol to sterilize our gloved hands
- Open round bottom tube, open container of autoclaved broth and open plastic for pipet
- Transfer 5ml of autoclaved broth without touching any of the containers
- pipet should be not used more than once
- Torch the top portion of the autoclaved broth container and quickly torch the inside of the autoclaved broth top
- Close autoclaved broth container
- Dip metal loop into ethanol and torch, allow to cool for 30 seconds
- Open NaaBlue Cells
- Dip burned loop into cells and then dip the loop into the 5ml of broth and stir
- Torch the loop to kill off any bacteria
- Close up NaaCells and sore in freezer
- Solution should be cloudy with cells after 24 hours of incubation at room temperature.
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