User:Tara K. Luckau/Notebook/Team ConGen/2010/12/03

Failed Gel on Scun22

• power supply started smoking ...
• found different power supply, but no bubbles
• borrowed a rig from Bohonak's lab, but not very many bubbles
• made new TAE buffer (had been re-using the buffer, this was approx the 5th gel), but no dye migration
• made new agarose gel from new TAE buffer with ladder and dye, ran on borrowed rig with our power supply, nada
• brought rig with gel and buffer to Bohonak's power supply - dye migration within 5 minutes.

Conclusion: find a new power supply that works.

Ugh.