User:Tara K. Luckau/Notebook/Team ConGen/2011/03/04

Scun22 Optimization PCR Gel

• from 23 february 2011 PCR (full temperature and buffer gradient)

Gel Specs

• Pour: 270mL 1xTAE + 5g agarose (supposed to have been 5.4g) + 27µL GelRed
• Run: 40 min @ 110V

Gel Images

• Only Buffer G yielded any product ... zoom in:
• 

Conclusions

• doesn't look like a typical MgCl₂ and temperature gradient PCR
• inconsistent banding patterns across temperatures with dropouts

• regardless, there is mispriming in all conditions that yielded any product
• Scun22 might not be a primer that will work for Sceloporus occidentalis