User:Tara K. Luckau/Notebook/Team ConGen/2011/03/04

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Scun22 Optimization PCR Gel

  • from 23 february 2011 PCR (full temperature and buffer gradient)

Gel Specs

  • Pour: 270mL 1xTAE + 5g agarose (supposed to have been 5.4g) + 27┬ÁL GelRed
  • Run: 40 min @ 110V

Gel Images

  • Only Buffer G yielded any product ... zoom in:


  • doesn't look like a typical MgCl2 and temperature gradient PCR
  • inconsistent banding patterns across temperatures with dropouts

  • regardless, there is mispriming in all conditions that yielded any product
  • Scun22 might not be a primer that will work for Sceloporus occidentalis

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