User:Tara K. Luckau/Notebook/Team ConGen/2011/06/01

Scun3

Gel

Pour

• 270 mL 1x TAE + 5.4 g agarose
• microwave: set for 5 min @ half power, will first boil at 1:20, done about 0:20
• 27 µL GelRed

Load

• 1 µL gel load dye + 5 µL PCR product
• 6 µL ladder

Run

• 160 V
• 1 hour

Image