|
Scun10
Gel
| Pour | Load | Run
|
| 270 mL 1x TAE + 5.4 g agarose | 1 µL gel load dye + 5 µL PCR product | 160 V
|
| 27 µL GelRed | 6 µL ladder | 1 hour
|
- double banding noticeable under majority of PCR conditions; I'm assuming this is attributable to diploid nuclear DNA - in this case, a large enough difference in repeats between the two copies that it's clearly visible on an agarose gel
-
Scun11
PCR
Gel
| Pour | Load | Run
|
| 270 mL 1x TAE + 5.4 g agarose | 1 µL gel load dye + 5 µL PCR product | 140 V
|
| 27 µL GelRed | 6 µL ladder | 1.5 hour
|
Scun14
PCR
|
Tara's Lab Notebook
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