User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06

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ASHY Multiplex

  • prepping to start multiplexing CNHY primers ... so see primer interactions ...

Gradient Summary

  • green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
  • Image:20120106 GradientSummary.png

Multiplex Map

  • markers placed arbitrarily
  • marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
  • Image:20120106 Map.png


  • color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions

PCR - determine approximate range of each marker

  • pool high-quant DNA samples from throughout SD county:
-- CNHY CPN 02
-- CNHY LOM 08
-- CNHY RAJ 01
-- CNHY RAJ 35
-- CNHY RAJ 42
-- CNHY RAJ 50
-- CNHY TP1 11
-- CNHY TP3 05
-- CNHY TP3 19
-- CNHY TP3 20
  • 2µL each DNA STK + 80µL LowTE (to dilute to ~10ng/µL) + 20µL CNHY 062911TKLH ('play' DNA, already 10 ng/µL)

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