User:Tara K. Luckau/Notebook/Team ConGen/2012/01/13

CNHY / CNTI Multiplex Planning

Primer Interactions

• from 2012/01/06 AutoDimer, interactions of concern are:

Revised Multiplex Map

• revised amplicon size ranges based on uniplex PCRs run on agarose gel (2012/01/09)

Conclusions

• initially, would suggest three separate multiplexes (to separate Acos3 from Ai5057, both of which overlap with Ai5013

• decided to try to cram into 2 multiplexes (and ignore the Acos3-Ai5057 interaction)
• 

• MultiplexA:

Ai5013, Ai5033, Ai5043, Ai5062, Ai5071

Buffer E, 56°C

• MultiplexB:

Acos3, Acos5, Ai5035, Ai5037, Ai5057

Buffer F, 56°C

• NEXT: design set of PCRs to send to frag

SCOC MPY

PCR

• using un-pigtailed sar80-R (because sar80-R-pig dimerizes heavily with sar-80-NED) 2011/10/21 Conclusions and Next Steps
•