User:Tara K. Luckau/Notebook/Team ConGen/2012/01/17

Tara's Lab Notebook

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CNHY / CNTI Multiplex & SCOC MPY sar80 Pour Load Run 270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 120 V 27 µL GelRed 6 µL ladder 25 minutes only used 1 row; saved rest of gel for later use this is just confirmation gel, so not all samples were run on gel for CNHY / CNTI gel lanes, used CNHY062911TKLH Uniplex: A1-7 Multiplex all-in-one: A8-9 Multiplex A & B: G1, G4 for SCOC gel lanes, used first three G7-9 (to include water) Gel - SCOC MPY sar80 trial water is clean two bands visible, in range of Scun3 (200bp) and sar80 (300bp) (but Scun11 band also in 300bp range) hoping all three amplicons are visible in frag analysis Gel - CNHY / CNTI Uniplexes some uniplexes didn't seem to amplify not gunna worry about it, since amplification successful in previous uniplex (2012 January 9 Uniplex gel) assuming other samples in this PCR had successful amplification Gel - CNHY / CNTI Multiplex all-in-one only see one prominent band in the 200bp range very strong primer banding (inefficient PCR)  :( Gel - CNHY / CNTI Multiplex A and Multiplex B no strong bands, only faint banding bands do seem to be different between MPA and MPB very strong primer banding (inefficient PCR)  :( good enough - send to frag! Fragment Analysis Submission FedEx Tracking# 876927708729 UAGC Submission# TSSB FedEx picked up 5:18pm, 17 January FedEx delivered 9:22am, 19 January UAGC received 2:52pm, 19 January UAGC completed 11:00am, 20 January